Enzimas de Restrição
Restriction endonucleases can be considered as the set of "working horses" aimed to point out specific sequences, to produce "sticky ends", to provide DNA fragment isolation and cloning. In spite of the successful replacement of restriction endonucleases by PCR-techniques in some applications, these enzymes still have rather high potential for DNA manipulations. A lot of procedures like mutation assays, mapping and cloning can hardly survive without restriction endonucleases even today.
Available Restriction endonucleases: