V.R.B.A. with Glucose & Lactose is a medium recommended for the detection and enumeration of enterobacteria, formulated according to European Pharmacopoeia.
Pancreatic Digest of Gelatin provides nitrogen and other nutrient to support microbial growth. Yeast Extract provides amino acids and vitamins of group B. Sodium Chloride maintains the osmotic balance of the medium. Bile Salts and Crystal Violet inhibit Gram-positive bacteria while lactose and glucose are a source of energy which allows the differentiation of microorganisms. Neutral Red is a pH indicator. Agar is the solidifying agent.