Slanetz Bartley Agar (m-Enterococcus A.) is a selective medium for enterococci enumeration and isolation in water and foods by membrane filtration technique or by direct plating, prepared according to the formulation reported by ISO 7899-2.
Tryptose is a mixed enzymatic hydrolysate with distinctive nutritional properties and provides nitrogen, amino acids and vitamins in culture medium. Glucose is a source of energy. Yeast extract is a source of amino acids and vitamins of group B. Dipotassium hydrogen phosphate acts as a buffer for the medium. Sodium azide is the selective agent to suppress the growth of gram-negative organisms. Agar is the solidifying agent. The 2,3,5-triphenyl tetrazolium chloride is reduced to the insoluble formazan inside the bacterial cell, resulting in the production of red colonies.