Antibiotic residue detection in urine and other biological fluids by Bacillus subtilis.
Antibiotic Test is a device constituted by particular paper discs impregnated with living spores of Bacillus subtilis ATCC 6633 and with a dry culture medium. This device is indicated for the evaluation of residual antimicrobial power in urines, milk and other biological fluids.
The paper disk contains living spores of Bacillus subtilis ATCC 6633 and a culture medium with triphenyl- tetrazolium- chloride (TTC). Spores and medium are released into solution after the addition of the liquid sample to examine (urine, milk or other biological fluid). Substances endowed with antimicrobial activity, eventually present in the liquid sample, inhibit Bacillus subtilis spores development, so that liquid in the tube does not change its colour. In the case of lack of substances with antimicrobial activity, Bacillus subtilis spores sprout. Vegetative forms multiply in the culture medium and determine the development of a red colour in the sample for the reduction of TTC to formazan, which is a red compound.
Each kit contains:
1 cartridge with 50 discs each, impregnated with living spores of Bacillus subtilis ATCC 6633 and with a dry culture medium;
50 plastic tubes;
1 instructions sheet.
The development of a red color in the tube of the sample indicates the absence of antimicrobial substances or their presence at a lower concentration than sensitive limit of the methods.
The absence of a red colour in the tube indicates the presence of antimicrobial substances in the sample at a higher concentration than sensitive limit of the method.
|Antibiotic Test (50 discos)||80350||***|
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