Eukaryotic transcription is a complex and tightly regulated process of generating an RNA template from a gene. The formation of the transcription initiation complex to begin the process is controlled by many gene-specific transcription factors and polymerase machinery. Because eukaryotic genes are wound around histones, histones and their modifications also play an important role in controlling gene expression.
DNA that is tightly packed into a complex with core histones in an ordered nucleosome structure has been termed heterochromatin, and has been thought to be less actively transcribed than DNA that is more accessible to activating proteins. Traditional methods for screening DNA-binding proteins include electrophoretic mobility shift assays (EMSA) and reporter assays. As research into gene expression and regulation have enabled many new discoveries to be made about the importance of protein modification and the overall structure of the DNA, new methods such as chromatin immunoprecipitation (ChIP) and 3C have been applied to the study of transcriptional regulation.
From simple transcription-factor DNA binding ELISAs to the most sensitive luciferase reporter assays, Active Motif has the reliable kits you need for consistent results in transcription factor research. Click on the links to the right for more information.