In vitro transcribe RNAs from dsDNA templates containing the T7 RNA polymerase promoter
- Produce RNA transcripts by in vitro transcription using this enzyme with any dsDNA template containing a T7 promoter
- Use for a variety of molecular biology applications requiring in vitro synthesize RNA.
The T7 RNA Polymerase produces defined RNA by in vitro transcription of dsDNA that is downstream of the specific T7 RNA polymerase promoter. This enzyme preparation is highly purified, and exhibits excellent promoter specificity.
- Synthesis of RNA for nucleic acid amplification methods or gene expression studies.
- RNA synthesized can be used as: a hybridization probe, antisense RNA, a ribozyme, a template for in vitro translation, a precursor mRNA for splicing or other processing studies, or to make dsRNA for RNA interference or gene silencing.
Unit Definition: One unit of RNA polymerase catalyzes the incorporation of 1 nmol of ribonucleoside triphosphate into RNA in 1 hour at 37°C under standard assay conditions using a DNA template with the appropriate T7 promoter.
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1.0 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.
Quality Control: The T7 RNA polymerase is tested in in vitro transcription reactions, and is free of detectable exo- and endonuclease and RNase activities, and E. coli RNA polymerase activity.
|T7 RNA Polymerase (50,000U|1,000U/ul)||TH950K||***|
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