Biologia Molecular Epicentre Kits Extração/Purificação DNA/RNA
Proteinase K
Descrição

Proteinase K is a serine protease active against a wide variety of proteins. Proteinase K is stable and active in SDS and urea and is useful for a variety of applications such as purification of nuclease-free plasmids, high molecular weight genomic DNA and intact RNA.

 

Applications

  • Isolation of plasmids and high molecular weight genomic
  • DNA Isolation of intact RNA
  • Inactivation of RNases, DNases and enzymes in molecular biology reactions

 

Benefits

  • Supplied in solution at 50 µg/µl
  • Function tested for purification of high molecular weight genomic DNA and intact RNA

 

Proteinase K is active under a wide range of temperature and buffer conditions.

  • Temperature Range:
  • Room temperature to 60°C
  • pH Range: pH 4 to 12
  • Calcium: 1 – 5 mM Ca+2
  • Protein Denaturants: Active under denaturing conditions such as 0.5% – 1% SDS or 1 M – 5 M urea

 

Concentration: Supplied in solution at 50 µg/µl

Storage Buffer: 50% glycerol, 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1mM EDTA, 10 mM CaCl2, 0.1% Triton X-100 and 1 mM DTT

Store: -20°C

Stability: Guaranteed to retain full activity for 12 months from date of purchase when stored at -20°C in the original container.

Function Tested: Proteinase K is function tested in purifying high molecular weight DNA or RNA using the Epicentre MasterPure™ DNA and RNA Purification Kit.

 

Nota: Os produtos que necessitam de transporte em condições refrigeradas, têm custos adicionais. Por favor, contacte-nos.

Descrição Referência Preço Quantidade
Proteinase K, 50 µg/µl (2ml) MPRK092 ***
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