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OmniCleave Endonuclease
Descrição

Remove all types of RNA and DNA with this robust, flexible nuclease.

 

Non-specific: Digests all types of DNA and RNA including single- or double-stranded linear, circular, and supercoiled

Flexible: Active within a broad range of conditions normally used in protein purification

Customizable: Contact us for bulk or high concentration enzyme.

 

OmniCleave™ Endonuclease is a highly purified enzyme from a recombinant E. coli strain that degrades single- and double-stranded DNA and RNA to di-, tri-, and tetranucleotides. OmniCleave Endonuclease has the same substrate specificity and yields the same products as Benzonase®, an enzyme derived from Serratia marcescens.

Especificações

Applications:

  • Removal of nucleic acids from cell lysates (reduction of viscosity) for improved handling and yield of protein preparations.
  • Removal of trace contamination by nucleic acids in protein preparations.
  • Removal of host DNA from phage preparations.
  • Improved electrophoretic and chromatographic separation of proteins isolated from whole-cell lysates.

 

Unit Definition: One unit of OmniCleave Endonuclease converts 1.0 A260 (~60 µg) of sonicated salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in 50 mM Tris-HCl (pH 8.0 at 25°C) and 1 mM MgCl2.

 

Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5 at 25°C), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.

 

Quality Control: OmniCleave Endonuclease is free of detectable protease activity.

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OmniCleave Endonuclease (50,000U) OC7850K ***
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