MyGo ProTaq Red Mix is a high-performance mix designed for the amplification of difficult templates. The mix includes MyGo ProTaq Polymerase, dNTPs, MgCl2, and a red tracking dye in an optimised buffer designed to give the best results under challenging conditions such as the amplification of high GC targets and the presence of inhibitors. The tracking dye enables direct loading of PCR products onto agarose gels.

MyGo ProTaq Polymerase uses antibodymediated hot start to give fast, highly specific PCR. The enzyme is inactive at ambient temperatures, preventing the formation of primer-dimers and mis-primed products with the convenience of room temperature setup. The enzyme is activated at the start of a reaction with a 95°C incubation step. The enhanced efficiency and specificity of MyGo ProTaq Polymerase make it the ideal choice for the accurate amplification of long and complex templates.

MyGo ProTaq Red Mix is ready-to-use and supplied in a single tube containing all the reagents required for trouble-free PCR setup. It is recommended for the amplification of human genomic DNA up to 20kb. MyGo ProTaq Polymerase exhibits 2.5 fold higher fidelity than Taq DNA Polymerase. PCR products generated are A-tailed, and may be cloned into TA cloning vectors.