MyGo Probe 1-Step contains all the reagents required for rapid and sensitive cDNA synthesis and subsequent real-time PCR in a single tube.

The kit has been designed for use with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. It includes a thermostable reverse transcriptase and 2x MyGo Probe 1-Step Mix containing MyGo HS Taq DNA Polymerase, dNTPs and MgCl2 optimised to give the best results under challenging conditions such as high GC or low-copy RNA targets.

The kit uses a thermostable modified Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase optimised for reliable cDNA synthesis over a wide dynamic range of input RNA. The enzyme is provided preblended with RNase inhibitor to prevent degradation of RNA by contaminating RNase.

MyGo Probe 1-Step Mix uses antibodymediated hot start to provide highly specific and sensitive amplification. MyGo HS Taq DNA Polymerase is inactive at ambient temperatures, preventing the formation of primer-dimers and mis-primed products with the convenience of room temperature setup.