MyGo 1-Step RT-PCR Kit contains all the reagents required for rapid and sensitive cDNA synthesis and subsequent PCR in a single tube. The kit includes a thermostable reverse transcriptase and 2x MyGo 1-Step Mix containing MyGo HS Taq DNA Polymerase, dNTPs, and MgCl2 optimised to give the best results under challenging conditions such as high GC or low-copy RNA targets.

The kit uses a thermostable modified Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase optimised for reliable cDNA synthesis over a wide dynamic range of input RNA. The enzyme is provided preblended with RNase inhibitor to prevent degradation of RNA by contaminating RNase.

MyGo 1-Step Mix uses antibody-mediated hot start to provide highly specific and sensitive 1-Step RT-PCR. MyGo HS Taq DNA Polymerase is inactive at ambient temperatures, preventing the formation of primer-dimers and mis-primed products with the convenience of room temperature setup.