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Exonuclease I
Description

Digest ssDNA, but not dsDNA, in a 3´ to 5´ direction

  • Specific: Processively digest ssDNA in a 3´ to 5´ direction without harming dsDNA - short 3´ overhangs in DNA are not digested
  • Flexible: Active under a wide variety of buffer conditions and can be added directly into most reaction mixes
  • Heat Inactivated: Destroy the enzyme activity by incubating at 80°C for 15 minutes.

 

Exonuclease I digests ssDNA in a 3´ to 5´ direction,1-3 but does not digest dsDNA. Although it requires the presence of magnesium and a free 3´-hydroxyl terminus for activity, it is active under a wide variety of buffer conditions and can be added directly into most reaction mixes. Exonuclease I can be heat-inactivated by incubation at 80°C for 15 minutes.

Specifications

Applications:

  • Removal of residual ssDNA, including oligos, from reaction mixes.
  • Removal of ssDNA from nucleic acid mixtures.

 

Unit Definition: One unit of Exonuclease I catalyzes the release of 10 nmol of acid-soluble nucleotides from heat-denatured calf thymus DNA in 30 minutes at 37°C under standard assay conditions.

 

Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.

 

Quality Control: Exonuclease I is tested in degradation of ssDNA and is free of detectable RNase, endonuclease, and double-stranded exonuclease activities.

Downloads
Description Reference Price Quantity
Exonuclease I (20,000U) X40520K ***
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