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Episcript RNase H- Reverse Transcriptase
Description

An optimized reverse transcriptase system for the production of full-length cDNA.

 

  • Produce full-length cDNA with RNAse H- mutant
  • Achieve higher specificity at elevated temperatures, up to 55°C.

 

EpiScript™ Rnase H- Reverse Transcriptase (EpiScript RT), an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.

Specifications

Storage: Store only at -20°C in a freezer without a defrost cycle.

 

Storage Buffer: EpiScript RT is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM sodium chloride, 1 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.

 

Unit Definition: One unit of EpiScript RT catalyzes the incorporation of 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using saturating amounts of oligo(dT)-primed poly(rA) as template.

 

Contaminating Activity Assays: EpiScript RT is free of detectable exonuclease, endonuclease, and RNase activities.

Benefits
  • Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity
  • Active at temperatures up to 55°C
  • Highly efficient at producing full-length cDNA from as little as 50 pg of total RNA
  • Best value in an RNase H- Reverse Transcriptase
Downloads
Description Reference Price Quantity
Episcript RNase H- Reverse Transcriptase (10,000U) ERT12910K ***
Episcript RNase H- Reverse Transcriptase (25,000U) ERT12925K ***
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