The Chromatin Assembly Kit includes native HeLa core histones and recombinant chaperone and assembly proteins for the in vitro assembly of nucleosomes in a natural, ordered array on either circular or linear input DNA. The kit also includes reagents to confirm the ordered array of nucleosomes on the DNA with a simple and quick partial enzymatic digestion. The partial digestion clearly shows the ordered spacing of nucleosomes on the DNA.

Chromatin Assembly Kit advantages:

• Generate chromatin from linear or supercoiled DNA
• ATP-dependent method results in an extended array of regularly spaced nucleosomes
• Positive control supercoiled DNA provided to ensure success
• Easy protocol, simply add components and incubate
• Excellent substrate for gene regulation experiments

Applications

The chromatin generated with the Chromatin Assembly Kit can be used in many applications, such as investigation of factors involved in transcription activation by in vitro transcription assays. In addition, you can also determine which factors are involved with histone acetylation by performing in vitro histone acetylation (HAT) assays. You can also perform an in vitro ChIP assay of the assembled chromatin to verify transcription factor binding.

Contents & Storage

Recombinant hNAP-1 protein, purified HeLa Core Histones, recombinant ACF complex, high and low salt buffers, 10X ATP regeneration system, Creatine Kinase, control supercoiled DNA, Enzymatic Shearing Cocktail, Proteinase K and Enzymatic Stop solution, glycogen, 5X Orange G Dye, and siliconized microcentrifuge tubes. Storage conditions range from room temperature to -80°C, see the manual for specific details. Contents are guaranteed for six months from delivery when stored properly.

The purified HeLa Core Histones are also sold separately.