Blotting, a technique that entails immobilisation of proteins or nucleic acids on a solid membrane support and then detection using a specific antibody or probe of complementary nucleic acid sequence, significantly increases the potential for identification and characterisation of proteins and nucleic acids. Upon transfer to a membrane support proteins and nucleic acids become far more accessible to detection by antibodies and probes than they would otherwise be within a gel. Size-fractionation by gel electrophoresis followed by blotting is an excellent way to identify specific molecules within a mixed population of nucleic or protein molecules, and the two techniques are often used in tandem.
Frilabo offers tree types of system:
- Electroblotters – combine PAGE and transfer techniques within the same tank.
- Tank transfer systems – are ideal for protein identification. Full flexibility is offered in selecting voltage settings, blotting times, and cooling options. These systems, which are available with either plate or wire electrodes, support efficient, quantitative transfers over a wide molecular weight range. Plate electrode systems are faster through greater field strength, while wire electrodes are usually more economical, consuming less current and generating less heat.
- Semi-dry transfer systems – perfect for rapid, high-intensity transfers of mid-range proteins, 10-100kD in size.