Multi-Extraction reagent for DNA, RNA and Protein.

TriFaster is a complete ready to use reagent for the simultaneous isolation of RNA, DNA and proteins from liquid samples of human, animal, plant, yeast, bacterial and viral origin. The isolation method for RNA, DNA and proteins is built on the well-known single step liquid phase separation.

This method has been shown to yield undegraded RNA from small and large samples. Also it makes possible to process a large number of samples simultaneously.

TriFaster includes phenol and guanidinium thiocyanate in a monophasic solution. After addition of chloroform the homogenate separates into three phases upon centrifugation. The aqueous phase containing the RNA, the interphase the DNA and the organic phase the protein are processed separately resulting in RNA, DNA and protein of excellent quality in high yield. Simultaneous purification of DNA is an effective way to normalize RNA yields from sample to sample. Certain cells contain a material of unknown nature in the final RNA fraction. This material is readily soluble in water, insoluble in alcohol, forming DNA-like fibers and has an absorbance similar to nucleic acids in UV light. This material, most likely polysaccharides, is readily solubilized by guanidinium thiocyanate, seems not to interfere with hybridizations, but leads to wrong RNA estimations in the final preparations. In fact even cells in different developmental stages have been shown to contain different amounts of this contaminating material. A simple extension of the standard OptiPure procedure eliminates these impurities.

Features:

- extraction of RNA, DNA and proteins from the same sample - for small and large amounts of probes

- simultaneous preparation of many samples

- for fresh, lyophilized and frozen samples

- highest yield of non-degraded total-RNA

- short precipitation time

Applications:

- RT-PCR and PCR

- Restriction digestion, ligation

- Northern and Southern blotting

- Poly(A)+ - RNA (mRNA) purification

- Nuclease protection assays

- In-vitro translation

Yield:

1 – 15 μg RNA per mg tissue

2 – 7 μg DNA per mg tissue

0.2 – 13 μg per mg protein

WARNING: TriFaster contains phenol and guanidinium thiocyanate. This combination can be fatal. During work with TriFaster gloves and eye protection should be used. In case of contact with skin or eyes etc. wash excessively with water for at least 15 minutes and seek medical care. Work in a hood.

Storage: +4°C

Transportation: room temperature