The Plasmid DNA Extraction Kit is designed for rapid and efficient purification of high copy and low copy plasmid DNA from bacterial lysates.

The GF-1 Plasmid DNA Extraction Kit is designed for rapid and efficient purification of high copy and low copy plasmid DNA from bacterial lysates. This kit uses the alkaline lysis-SDS method to lyse cells and release plasmid DNA. Special buffers provided in the kit are optimized to enhance binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure plasmid DNA.

Features:

- Yields up to 20µg of DNA

- Multiple samples can be processed rapidly in less than 30 minutes

- No organic-based extraction required

- Highly pure plasmid DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, DNA sequencing, ligation, transformation, etc.

Application: For high quality plasmid DNA isolation.

Message: Plasmid DNA Extraction Kit is designed for rapid and efficient purification of high copy and low copy plasmid DNA without the need for precipitation or organic extractions. This it uses a specially-treated glass filter membrane fixed into a column to efficiently bind DNA in the presence of high salt. Combining alkaline lysis-SDS and minicolumn spin technology, up o 20μg of plasmid DNA from bacterial cultures can be isolated.

Multiple samples can be processed rapidly and with practice, the purification takes less than 30 minutes. Optimized buffers ensure only highly pure plasmid DNA is extracted and is ready to use in many routine molecular biology applications such as restriction enzyme digestion, radioactive/fluorescence DNA sequencing, PCR, ligation, transformation and other manipulations.