Bacteriophage T7 DNA is isolated from an infected E. coli strain. DNA is linear, 39937 bp long. Some restriction enzymes have no recognition sequences on lambda DNA, therefore these enzymes are to be analyzed on the other DNA substrate. T7 DNA is the most popular “alternative” to Lambda DNA substrate for the restriction endonucleases. The complete nucleotide sequence has been determined.

Storage buffer:

• 10 mM Tris-HCl (pH 7.8)

• 10 mM NaCl

• 1 mM EDTA

Storage conditions: -20°C