The PCR Clean Up Kit is a system designed for rapid clean up of DNA bands ranging from100bp to 20kb.

The GF-1 PCR Clean-up Kit is designed for rapid and efficient clean up of DNA ranging from 100bp to 20kb. The kit efficiently removes dNTPs, short oligo fragments, mineral oil, enzymes from a PCR reaction product, removes proteins after restriction enzyme treatment and dephosporylation and residual dye and ethidium bromide. This kit is also suited for concentrating DNA, changing of buffers and desalting.

Features:

- Indicator dye for easy pH determination

- 90% of recovery achievable

- Mini-column spin technology

- Purification process less than 15 minutes

- Highly pure DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, ligation, DNA sequencing, probe preparations, etc.

Application: For purification of DNA from reaction solutions.

Message: The PCR Clean Up Kit contains special buffers to provide the correct salt concentration and pH for efficient recovery (80 - 90%) of DNA. This kit uses a specially treated glass filter membrane fixed into a column to efficiently bind DNA in the presence of high salt. It applies the principle of a mini-column spin technology and is well suited for the removal of excess dNTPs, short oligo fragments, mineral oil, enzymes from a PCR reaction product, proteins after restriction enzyme treatment and dephosporylation and residual dye and ethidium bromide. This kit also allows for concentration of DNA, changing of buffers and desalting. Buffer PCR contains a pH indicator for easy determination of the optimal pH for efficient recovery of DNA fragments but at the same time does not interfere with DNA yield and quality as it is thoroughly removed during the subsequent washing steps. High recovery of pure DNA obtained is ready-to-use in many routine molecular biology applications such as restriction enzyme digestion, radioactive/fluorescence DNA sequencing, PCR, ligation and transformation, probe preparations and other manipulations.