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Accura High-Fidelity Polymerase
Description

Accura™ High-Fidelity Polymerase is the perfect solution for your everyday amplification and cloning needs. Amplify your genes for cloning and protein expression applications with confidence while getting the most out of your laboratory budget. The Accura High-Fidelity polymerase is a fusion protein, consisting of a DNA binding domain and a unique, thermostable polymerase derived from a bacteriophage. When used according to recommendations, Accura High-Fidelity polymerase results in robust amplification of normal, high GC% or otherwise difficult templates.

Specifications

Concentration: 2 units/µl.

Absence of Endonuclease or Nicking Activity: Incubation of 2 U of Accura™ High-Fidelity Polymerase with 1 µg of supercoiled pUC19 DNA for 16 hours at 37°C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis.

Absence of Exonuclease Activity: Incubation of 2 U of Accura™ High-Fidelity Polymerase with 1 µg of HindIII-cut lambda DNA for 16 hours at 37°C resulted in no smearing of bands on agarose gels.

Benefits
  • High-Fidelity: Ideal for cloning and protein expression
  • Flexibility: HF and GC buffers included to handle even the toughest targets
  • Performance: Robust amplification and sensitivity
  • Price: The most cost effective high-fidelity polymerase available
Downloads
Description Reference Price Quantity
Accura High Fidelity Polymerase (100U) 30010-1 ***
Accura High Fidelity Polymerase (500U) 30010-2 ***
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